molecular epidemiology is an offshoot of molecular genetics.here genetic methods such as
plasma profile analysis,genomic fingerprinting,and PCR are used to
isolate and match the microbial isolates for epidemiological purposes
two methods used are
1.phenotype
2.genotype
the methods for phenotype are
1.immunoblotting and electrophoretic typing of bacterial proteins
the methods for genotype are 1.plasma profile analysis
2.restriction endonuclease analysis of chromosomalDNa with southern blotting
3.polymerase chain reaction
and nucleotide sequence analysis
NORMALLY ALL OF MY POSTS CAN BE UNDERSTOOD EVEN BY A LAYMAN.BUT THIS
POST IS EXCLUSIVE ONLY FOR DOCTORS
Subscribe to:
Post Comments (Atom)
This comment has been removed by the author.
ReplyDeleteHi Doc. I am fascinated by this technology. The genebank has grown very large, and continues to be updated. Good way to track viral and bacterial mutations as well.
ReplyDeleteIs the testing sensitive enough to detect intracellular foreign DNA? As I understand it, the method targets mtDNA, thus increasing sensitivity. Do you think running a profile on mixed blood could isolate a pathogen of interest? I guess you would have to first characterize the host DNA. Know of any labs doing this at affordable cost?